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  • Title: Separation of lipase-positive cells from suspensions of pancreas cells in an isokinetic gradient of Ficoll in tissue culture medium.
    Author: Blackmon J, Pitts A, Pretlow TG.
    Journal: Am J Pathol; 1973 Sep; 72(3):417-26. PubMed ID: 4125699.
    Abstract:
    Lipase-positive cells were separated from suspensions of pancreas cells both by velocity sedimentation and by isopycnic sedimentation. Lipase-positive cells were 51.0 +/- 9.6% of the disaggregated pancreas cells in the starting sample suspension. The purest gradient fractions from experiments using velocity sedimentation for cell separation contained a mean of 98.8 +/- 0.6% lipase-positive cells. Cell separation using isopycnic centrifugation was less effective than cell separation using velocity sedimentation and resulted in a mean purity of only 89.8 +/- 6.8% lipase-positive cells. Lipase positivity was assessed using the Gomori technic with the conditions for fixation slightly modified to be suitable for disaggregated cells.
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