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  • Title: Conditions for using DNA polymerase I as an RNA-dependent DNA polymerase.
    Author: Gulati SC, Kacian DL, Spiegelman S.
    Journal: Proc Natl Acad Sci U S A; 1974 Apr; 71(4):1035-9. PubMed ID: 4133845.
    Abstract:
    Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybrids formed with the complementary DNA are of comparable specificity and stability to those formed with complementary DNAs synthesized by viral RNA-directed DNA polymerase. Synthesis of dA-dT polymers, a common occurrence with this enzyme, can be eliminated by including distamycin in the reaction mixture.
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