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Title: A simple method for the preparation of pure and active gamma-globulin-ferritin conjugates using glutaraldehyde. Author: Siess E, Wieland O, Miller F. Journal: Immunology; 1971 May; 20(5):659-65. PubMed ID: 4137515. Abstract: A method is described for coupling γ-globulin to ferritin using glutaraldehyde. Isolation of the conjugate is achieved by ammonium sulphate precipitation. The conjugate is free from uncoupled ferritin or γ-globulin and the coupled antibody retains full activity. Preparation of pure active conjugate takes about 2½ hours.[Abstract] [Full Text] [Related] [New Search]