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  • Title: Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.
    Author: Venetianer P, Leder P.
    Journal: Proc Natl Acad Sci U S A; 1974 Oct; 71(10):3892-5. PubMed ID: 4139705.
    Abstract:
    With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.
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