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  • Title: In vitro system for production of mouse mammary tumor virus.
    Author: Fine DL, Arthur LO, PLOWMAN JK, Hillman EA, Klein F.
    Journal: Appl Microbiol; 1974 Dec; 28(6):1040-6. PubMed ID: 4141598.
    Abstract:
    An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C(3)H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 x 10(10) particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.
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