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  • Title: [Ram sperm survival during the use of various solvents after deep freezing in liquid nitrogen].
    Author: Mesáros P, Gamcík P, Schvarc F.
    Journal: Vet Med (Praha); 1977 Oct; 22(10):599-604. PubMed ID: 414407.
    Abstract:
    Suitability of three diluents for a deep-freezing of ram ejaculates and sperm survival after de-freezing and in the course of a six-hour incubation were investigated. The longest survival was found with the use of diluent containing Tris, fructose, and citric acid: 49.2% after de-freezing and 20.7% after six hours. The influence of the de-freezing procedure on the motility of spermatozoa can also be taken into consideration. The best proved rapid de-freezing without any diluent. The stated results serve for orientation only as the suitability of the diluents will be confirmed by the fertility of inseminated ewes.
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