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  • Title: Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods.
    Author: Beaufay H, Amar-Costesec A, Feytmans E, Thinès-Sempoux D, Wibo M, Robbi M, Berthet J.
    Journal: J Cell Biol; 1974 Apr; 61(1):188-200. PubMed ID: 4150488.
    Abstract:
    The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b(5) and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-beta-glucosaminidase, beta-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.
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