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  • Title: The applicability of anaphylactic tests in studies of antigen mixtures. II. The discriminatory capacity of the tests.
    Author: Dale MM.
    Journal: Immunology; 1965 May; 8(5):444-53. PubMed ID: 4158139.
    Abstract:
    Some assumptions underlying the attribution of a high discriminatory capacity to anaphylactic tests have been investigated in model systems, using two tests of the anaphylactic response—the Dale—Schultze reaction and the measurement of histamine released from chopped lung. It has been demonstrated that there may be no response to challenge with a second antigen after desensitization to the first even though the tissues can be shown to be sensitized to the second antigen, i.e. there is a possibility of the occurrence of `false' negative results. The potentiality of response to the second antigen appears to depend partly on the proportion it formed of the sensitizing mixture and partly on the absolute dose used in sensitization. It has been confirmed that desensitization is a graded phenomenon related to dose of antigen and it is shown that it is reversible with time. These factors are likely to complicate the performance and/or interpretation of experiments with tissue extracts and constitute a source of potential false positive results. These factors are of less importance with the histamine-release test than with the Dale—Schultz test. For the Dale—Schultz reaction the ileum proved to be a more satisfactory test tissue than the uterus. In general, there was no significant difference in discriminatory capacity between the histamine-release test and the Dale—Schultz test, but there were more sources of error in the performance of the Dale—Schultz test, while the histamine-release test though more laborious to do gave results which were more amenable to statistical analysis. It is concluded that with careful experimental design the tests could possibly be of use in some investigations of antigen mixtures provided that the sources of false positive results were recognized and due precautions taken.
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