These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes.
    Author: Sasai Y, Kumano S, Takahashi M, Nakano H.
    Journal: Histochemistry; 1978 Apr 04; 55(3):235-43. PubMed ID: 417051.
    Abstract:
    The graphical method of Lineweaver-Burk was applied to the histochemical system to obtain quantitative information on the dopa-oxidase activity in human epidermal melanocytes. In vitro experiment showed that the incubation time necessary for yielding a certain amount of dopa-melanin is related to the amount of enzyme present. In the case of histochemical experiment, the time required for the first appearance of dopa-melanin deposition in melanocytes at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the dopa-oxidase activity at various sites of the tissue.
    [Abstract] [Full Text] [Related] [New Search]