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  • Title: Studies in cryo-immumology. 3. The immunoglobulin nature of the antibody response.
    Author: Shulman S, Bronson P, Riera C, Brandt EJ, Yantorno C.
    Journal: Immunology; 1968 Apr; 14(4):541-51. PubMed ID: 4172527.
    Abstract:
    Antisera taken at various intervals over a 21-day period, from six rabbits subjected to cryosurgery of the accessory glands, were passed through columns of Sephadex G-200. The appropriate pools were made, adjusted to the initial serum volumes, and tested by tanned cell haemagglutination for antibody to rabbit accessory glands antigen. In every instance it was possible to demonstrate a predominant 19S activity in the bleedings obtained at 5–8 days; in most of the rabbits the presence of 7S activity could be observed in the later bleedings, and it was found to be the major form at these later stages in the immune response.
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