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  • Title: Detection of nuclease activity in semisolid and broth cultures.
    Author: Lachica RV, Deibel RH.
    Journal: Appl Microbiol; 1969 Aug; 18(2):174-6. PubMed ID: 4185235.
    Abstract:
    A method for the detection of deoxyribonuclease activity in semisolid agar cultures was investigated. When cultures were overlaid with an acridine orangedeoxyribonucleate-agar (ADA) mixture, incubated for 1 to 3 hr, and observed under ultraviolet light, clear halos developed around colonies that produced deoxyribonuclease. A variation of the method for use with broth cultures involved impregnation of filter-paper discs with a small portion of the culture and overlaying the discs with the ADA mixture. This alteration has the advantage that the test tube cultures are easily heat-treated prior to assay to determine the heat resistance of the enzyme.
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