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  • Title: Immunoglobulin peptide chain synthesis in the newborn rabbit.
    Author: Adler WH, Curry JH, Smith RT.
    Journal: Immunology; 1969 Sep; 17(3):457-68. PubMed ID: 4187457.
    Abstract:
    Lymphoid tissue from newborn rabbits was examined using fluorochrome conjugated goat antisera to rabbit γ, μ and α heavy chain and mixed light chain. Unimmunized newborn rabbits examined at intervals up to 20 days of age revealed a very low background of approximately one in 105 cells which stained with either the anti-μ, anti-γ or anti-L chain reagents. Newborn rabbits, immunized intraperitoneally on the day of birth with S. paratyphi B (4, 5, 12:b), showed a 1000-fold increase in the number of stained cells; μ heavy chain containing cells were found 16 hours after immunization, and γ heavy chain containing cells were found 20 hours after immunization in the rabbit spleen tissue. The numbers of μ containing cells and γ containing cells were approximately equal at 5 days of age in the immunized groups. No α heavy chain containing cells were found in any of the rabbits studied. All cells which stained with the anti-μ or γ heavy chain reagent were also stained with the anti-light chain reagent. The cellular response of the immunized newborn rabbits could be inhibited by passive antibody to S. paratyphi B, either given at the time of immunization, or passively acquired from the doe. Specificity of inhibition was indicated by the failure of sheep red blood cells (SRBC) stroma to be inhibited in animals so suppressed. The range of cellular morphology of the γ chain containing and the μ chain containing cells was indistinguishable. The earliest cell to be stained with either anti-γ or anti-μ was a large blast-like cell with a thin rim of cytoplasm and a large nucleus. A full range of forms between this and typical plasma cells appeared later in each case. These findings present a paradox since γG antibody to this antigen does not appear in the serum of such animals following γM class in the usual sequence characteristic of adult animals. Possible explanations of this paradox are explored.
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