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  • Title: A quantitative antibody-binding method for the determination of specific antibody within different immunoglobulin classes. Application to four Ig classes in the mouse.
    Author: Nash DR, Heremans JF.
    Journal: Immunology; 1969 Nov; 17(5):685-94. PubMed ID: 4187521.
    Abstract:
    A quantitative method for determining specific antibody in the different classes of immunoglobulins is described. The technique is based on the binding of all antibody present in an immune serum with antigen, and the subsequent precipitation of the bound immunoglobulins with a heterologous antiserum specific for the antigen employed. The concentration of each immunoglobulin class is determined by means of single radial immunodiffusion, before and after removal of its antibody-active portion. The accuracy of the measurements is essentially that of the immunodiffusion technique, and the whole analysis, applied to four immunoglobulin classes, requires only 0.06 ml of serum. The applicability of the technique has been tested using sera from mice immunized with ferritin by different routes. Antibody activity was predominant in the IgG1 and IgG2 immunoglobulin classes. However, measurable anti-ferritin activity in the IgM and IgA classes was also found in a limited number of sera.
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