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  • Title: Location of O-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria.
    Author: Tharanathan RN, Mayer H.
    Journal: Biochem J; 1978 May 01; 171(2):403-8. PubMed ID: 418776.
    Abstract:
    An attempt was made to localize a number of O-methyl sugars in lipopolysaccharides and antigenic polysaccharides isolated from photosynthetic bacteria and from cyano-bacteria. Methylation analysis with [2H3]methyl iodide as methylating agent was the method of choice. One has to differentiate between (lipo-)polysaccharides having only trace amounts (less than 1% of polymer dry weight) of O-methyl sugars and those having them in larger amounts (more than 4% of polymer dry weight). In the former case O-methyl sugars occupy either non-reducing or reducing terminals. When present in larger quantities they may be present as part of each repeating unit either in chain-linked or in terminal positions or in both. A possible role of O-methyl sugars in biosynthesis of O-chains, and their contribution to the lipophilic character of the cell surface are discussed.
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