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  • Title: Sucrase and lactase synthesis in suckling rat intestine in response to substrate administration.
    Author: Raul F, Simon PM, Kedinger M, Grenier JF, Haffen K.
    Journal: Biol Neonate; 1978; 33(1-2):100-5. PubMed ID: 418823.
    Abstract:
    The intestinal brush border disaccharidases separated by gel electrophoresis were studied after oral administration of a high sucrose or lactose diet to 11-day-old suckling rats during 3 days. Some modifications of the brush border protein and eyzyme patterns could be attributed to the effect of the basic diet: increase of glucoamylase, appearance of a weak sucrase activity and of a second molecular form of maltase. However, the specific action of a given disaccharide on the synthesis of the corresponding hydrolytic enzyme could be clearly demonstrated. Indeed, the electrophoretic pattern after sucrose or lactose feeding showed a marked increase of the protein bands corresponding to sucrase-isomaltase or lactase activities.
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