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  • Title: The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum.
    Author: Every D, Ashworth JM.
    Journal: Biochem J; 1973 May; 133(1):37-47. PubMed ID: 4198517.
    Abstract:
    The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.
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