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Title: Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system. Author: Andrews P, Kitchen BJ, Winzor DJ. Journal: Biochem J; 1973 Dec; 135(4):897-900. PubMed ID: 4204955. Abstract: From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.[Abstract] [Full Text] [Related] [New Search]