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  • Title: Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system.
    Author: Andrews P, Kitchen BJ, Winzor DJ.
    Journal: Biochem J; 1973 Dec; 135(4):897-900. PubMed ID: 4204955.
    Abstract:
    From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.
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