These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Further modification of a technique for radioimmunoassay of insulin.
    Author: Wilson RB, Martin JM.
    Journal: Can J Comp Med; 1971 Oct; 35(4):298-300. PubMed ID: 4260943.
    Abstract:
    Since our original report we adapted a modification of the immunoassay procedure that increased the sensitivity and reproducibility of the assay. The differences between the present procedure and the previous one are: (a) an initial 20 hour incubation period before the addition of the tracer insulin, and (b) incubation at 37 degrees C instead of 4 degrees C. The adapted method resulted in standard curves of similar slope for bovine, porcine, rhesus monkey, canine and human insulin. The validity of the assay for bovine insulin was established by recovery and dilution experiments.
    [Abstract] [Full Text] [Related] [New Search]