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Title: Histone deacetylation in nuclei isolated from hepatoma tissue culture cells. Inhibition by sodium butyrate. Author: Perry M, Nelson D, Moore M, Chalkley R. Journal: Biochim Biophys Acta; 1979 Feb 27; 561(2):517-25. PubMed ID: 427171. Abstract: Nuclei from hepatoma tissue culture (HTC) cells were isolated by standard methods and incubated in media commonly used for nuclease digestions (DNAase I and micrococcal nuclease) and for in vitro RNA synthesis. During the incubation, histones can be deacetylated from both control cells and cells treated with 6 mM sodium butyrate to enhance the levels of histone acetylation. Deacetylation of histone is much more apparent in nuclei isolated from sodium butyrate-treated cells. Inclusion of 6 mM sodium butyrate in the incubation medium effectively inhibits the endogenous deacetylase activity acting on histones H3 and H4, whereas sodium acetate at the same concentration has very little inhibitory effect.[Abstract] [Full Text] [Related] [New Search]