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  • Title: Nitrogenase activity of immobilized Azotobacter vinelandii.
    Author: Seyhan E, Kirwan DJ.
    Journal: Biotechnol Bioeng; 1979 Feb; 21(2):271-81. PubMed ID: 427263.
    Abstract:
    As part of a program to investigate the use of biological nitrogen fixation for fertilizer ammonia production, an investigation into the immobilization of the aerobic, nitrogen-fixing bacterium, Azotobacter vinelandii was undertaken. Immobilization was acaccomplished by adsorption onto an anionic exchange cellulose (Cellex E) with loadings as high as 10'' cells/g resin. Immobilized cell preparations were tested under both batch and continuous-flow conditions. Nitrogenase activities as high as 4200 nmol/min g resin were observed as measured by the acetylene reduction assay. Immobilized cells retained their activity for as long as 117 hr in a continuous-flow reactor. Activity loss appeared to be related to the development of a variant strain.
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