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  • Title: Murine hepatitis virus: effect on liver RNA.
    Author: Tsuji T, Hirschowitz BI, Sachs G.
    Journal: Science; 1968 Mar 01; 159(3818):987-90. PubMed ID: 4295120.
    Abstract:
    After infection of mice with hepatitis virus MHV3, the RNA in the liver undergoes changes. The fraction extracted with phenol at 0 degrees C does not alter. However, the fraction extracted with hot phenol at elevated pH (60 degrees C, pH 8.3) shows a 16S peak on sucrose-density-gradient centrifugation. This fraction shows actinomycin D-resistant incorporation of C(14)-orotic acid in infected but not in control livers-possible evidence of the RNA nature of MHV3.
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