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Title: Methods for detecting food-borne enteroviruses. Author: Herrmann JE, Cliver DO. Journal: Appl Microbiol; 1968 Oct; 16(10):1564-9. PubMed ID: 4300896. Abstract: A method previously reported for detecting virus in a model system composed of cottage cheese contaminated with coxsackievirus type A9 has been adapted to detecting selected strains of enteroviruses in a variety of foods. Bentonite is omitted and serum is added for extracting virus from low-protein foods. Samples of foods, usually 25 g, must contain at least 3 to 4 plaque-forming units for a 50% probability of detecting virus. Sensitivity in detecting echovirus type 6 was lower than that for the other viruses used. After extraction from potato salad, poliovirus type 2 was completely reactivated if it had been neutralized with coproantibody, but it was only partially reactivated if neutralized with hyperimmune rabbit serum.[Abstract] [Full Text] [Related] [New Search]