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  • Title: 25-hydroxycholecalciferol: direct effect on calcium transport.
    Author: Olson EB, DeLuca HF.
    Journal: Science; 1969 Jul 25; 165(3891):405-7. PubMed ID: 4307056.
    Abstract:
    The perfused small intestine from a vitamin D deficient rat exhibits about one-half the calcium transport of the intestine from a rat given vitamin D. These levels of calcium transport can be maintained for at least 4 hours. Addition of 2.5 micrograms of 25-hydroxycholecalciferol added to the vitamin D deficient intestine via the arterial blood perfusate induces a rise in calcium transport to +D levels within 2 hours. In contrast, 250 micrograms of vitamin D(3) given in the same manner has no effect on the calcium transport level overa 4-hour period. These data provide strong evidence that 25-hydroxycholecalci ferol represents the metabolically active form of vitamin D(3).
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