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  • Title: Separation of the bovine colostrum M-1 glycoprotein into two components.
    Author: Bezkorovainy A, Grohlich D.
    Journal: Biochem J; 1969 Dec; 115(4):817-22. PubMed ID: 4311442.
    Abstract:
    1. Two glycoproteins were isolated from the M-1 acid glycoprotein fraction of bovine colostrum. 2. The lighter glycoprotein had a molecular weight of 7200, contained about 28.4% of carbohydrate, and had an absorption maximum at 275nm. The heavier glycoprotein had a molecular weight of 12000, contained 39.0% of carbohydrate, and had no absorption maxima in the 240-300nm. range of the spectrum. 3. The carbohydrate moiety of both glycoproteins was removable from the polypeptide moiety under the conditions of the beta-elimination reaction. 4. Periodate oxidation experiments showed that sialic acid was linked to galactose in both proteins.
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