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  • Title: A protein binding assay for adenosine 3':5'-cyclic monophosphate.
    Author: Gilman AG.
    Journal: Proc Natl Acad Sci U S A; 1970 Sep; 67(1):305-12. PubMed ID: 4318781.
    Abstract:
    A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.
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