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  • Title: Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.
    Author: Dempsey WB, Ito H.
    Journal: J Bacteriol; 1970 Nov; 104(2):658-67. PubMed ID: 4321330.
    Abstract:
    At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The aminotransferase isolated from this mutant has been compared to that isolated from wild-type E. coli and found to have apparently normal affinity for pyridoxal 5'-phosphate, but reduced affinity for pyridoxamine 5'-phosphate.
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