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  • Title: Chicken gizzard: relation between calcium-activated phosphorylation and contraction.
    Author: Hoar PE, Kerrick WG, Cassidy PS.
    Journal: Science; 1979 May 04; 204(4392):503-6. PubMed ID: 432654.
    Abstract:
    Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large Ca2+-and Sr2+-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca2+- and Sr2+-activated tensions. Adenosine 5'-O (3'-thiotriphosphate) only in the presence of Ca2+ induced irreversible Ca2+-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of 32P from [gamma-32P]adenosine triphosphate into the myosin light chains.
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