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  • Title: Determination of urinary 4-pyridoxic acid using high performance liquid chromatography.
    Author: Gregory JF, Kirk JR.
    Journal: Am J Clin Nutr; 1979 Apr; 32(4):879-83. PubMed ID: 433815.
    Abstract:
    A high performance liquid chromatographic method is presented for the determination of the vitamin B6 metabolite, 4-pyridoxic acid, in urine. Urine samples are treated with trichloroacetic acid to precipitate protein. An aliquot of the supernatant is chromatographed using 0.033 M phosphate buffer containing 5% (v/v) methanol (pH 2.2), a fluorometric detector and a commercial reverse phase octadecylsilica column. The high precision of the method and the absence of interfering compounds have been demonstrated. This method provides a rapid, sensitive, and quantitative technique for the measurement of urinary 4-pyridoxic acid for use in metabolic and nutritional studies.
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