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  • Title: Isolation and classification of feline picornavirus and herpesvirus in Denmark.
    Author: Flagstad A.
    Journal: Acta Vet Scand; 1972; 13(4):462-71. PubMed ID: 4345192.
    Abstract:
    Seventeen viral isolates cytopathic for kitten kidney cells were isolated from 23 cats with symptoms of the respiratory disease feline viral rhinotracheitis or feline influenza. Five of these are classified tentatively as calicivirus, a member of the Picornavirus group, and 12 have the properties of the herpesvirus. Classification is based on the cytopathic effect in cell cultures and physico-chemical characteristics. The five isolates which are classified tentatively as calicivirus produced a quick cytopathic effect without formation of intranuclear inclusion bodies. The isolates were resistant to chloroform, were not inhibited by IDU, were labile at pH 4 and were not stabilized against thermal inactivation by molar MgCl2. The 12 isolates which seem to belong to the herpesvirus produced intranuclear inclusion bodies in cell cultures. They were sensitive to chloroform, and multiplication was inhibited by IDU. Antisera were produced by inoculating rabbits with calicivirus and cats with herpesvirus. The five isolates classified tentatively as calicivirus belong in one serotype, and the 12 isolates which seem to belong to the herpesvirus also belong in one serotype. I et materiale på 23 katte med symptomer på luftvejslidelsen rhinotracheitis eller influenza er der isoleret 17 virusisolater, der har cytopatisk effekt på nyrecellekulturer af killinger. Fem af disse er klassificeret som calicivirus tilhørende familien Picornavirus, og 12 har egenskaber karakteristiske for herpesvirus. Klassifikationen er baseret på de cytopatiske forandringer i cellekulturer og fysisk-kemiske forhold. De fem calicivirusisolater frembragte en hurtig cytopatisk forandring i cellekulturer uden dannelse af intranukleære inklusionslegemer. Disse isolater var resistente overfor kloroform, blev ikke hæmmet af IDU, var labile ved pH 4 og blev ikke stabiliseret af MgCl2 mod varmeinaktivering. De 12 herpesvirusisolater dannede intranukleære inklusionslegemer i cellekulturer. De blev inaktiveret af kloroform og hæmmet af IDU. De fem calicivirusisolater tilhørte én serotype og de 12 herpesvirusisolater én serotype.
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