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  • Title: Control of pantothenate accumulation in Agrobacterium tumefaciens.
    Author: Kaneshiro T, Arthur LO, Nickerson KW.
    Journal: J Bacteriol; 1973 Feb; 113(2):619-26. PubMed ID: 4347924.
    Abstract:
    Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. One of them (strain WMP-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. Extracts of cells grown on limiting aspartate were found to contain four times more (14)C-pantothenate than those grown at optimal aspartate concentrations. This difference was found in both the perchloric acid-soluble and -insoluble fractions, presumably the coenzyme A pool and acyl carrier protein, respectively. These findings are discussed in terms of membrane integrity and the control of fatty acid biosynthesis.
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