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  • Title: Measurement of plasma renin substrate by radioimmunoassay of angiotensin. I. Concentration in syndromes associated with steroid excess.
    Author: Krakoff LR.
    Journal: J Clin Endocrinol Metab; 1973 Jul; 37(1):110-7. PubMed ID: 4351771.
    Abstract:
    Plasma renin substrate (PRS) and plasma renin activity (PRA) were measured by radioimmunoassay of generated angiotension I in normal subjects and patients with steroid excess syndromes. In subjects with Cushing's syndrome, glucocorticosteroid therapy and oral contraceptive agents or estrogens, significant elevations of plasma renin substrate was observed. Only in subjects with primary aldosteronism was the plasma renin activity significantly supressed. The significance of elevations of PRS was assessed by determining the Km of the plasma renin-substrate reaction at physiologic pH. The apparent Km for the reaction was 1000 ng Angiotension I in PRS units. Correction of measured PRA to a standardized PRA was achieved by calculation of the reaction rate that would occur at the mean normal PRS concentration of 1100 ng/ml. Results indicate that elevations of PRS which occur in Cushing's syndrome or with administration of glucocorticoids, oral contraceptive agents, or estrogens are sufficient to significantly elevate the renin substrate reaction in plasma. In contrast, glucocorticoid excess appears not to suppress plasma renin activity even when reaction rates are corrected for increased PRS.
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