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  • Title: Membrane proteins of uninfected and Rous sarcoma virus- transformed avian cells.
    Author: Bussell RH, Robinson WS.
    Journal: J Virol; 1973 Aug; 12(2):320-7. PubMed ID: 4355934.
    Abstract:
    A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 x 10(5). MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.
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