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  • Title: Induction and catabolite repression of L-rhamnose dehydrogenase in Pullularia pullulans.
    Author: Vieira MM, Rigo LU, Maréchal LR, Veiga LA.
    Journal: J Bacteriol; 1979 Apr; 138(1):55-9. PubMed ID: 438135.
    Abstract:
    The growth of Pullularia pullulans on L-rhamnose (6-deoxy-L-mannose) as the sole carbon source induces the synthesis of L-rhamnose dehydrogenase, a nicotinamide adenine dinucleotide-dependent enzyme that catalyzes the oxidation of the deoxy sugar to L-rhamnonolactone. The enzyme induction is inhibited by cycloheximide, suggesting de novo synthesis. The presence of d-glucose (0.2%) or D-galactose (0.2%) simultaneously with the inducer in the induction medium produced 50% repression of dehydrogenase synthesis, but no effect was detected with D-fructose and D-mannose at the same concentration. High levels of D-glucose (2%), under maximal catabolite repression conditions, produced a complete inhibition of enzyme synthesis.
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