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  • Title: Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.
    Author: Miao R, Guild WR.
    Journal: J Bacteriol; 1970 Feb; 101(2):361-4. PubMed ID: 4391800.
    Abstract:
    The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.
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