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  • Title: Simplified determination of lorazepam and oxazepam in biological fluids by gas chromatography-mass spectrometry.
    Author: Higuchi S, Urabe H, Shiobara Y.
    Journal: J Chromatogr; 1979 Sep 11; 164(1):55-61. PubMed ID: 44299.
    Abstract:
    Lorazepam and oxazepam in plasma and urine were measured by gas chromatography-mass spectrometry. Oxazepam was used as an internal standard in the assay of lorazepam and vice versa. After removal of interfering substances with n-hexane, the drugs were extracted with benzene and converted to N1,O3-bistrimethylsilyl derivatives. Glucuronide forms of the drugs were extracted after hydrolysis with beta-glucuronidase. A common fragment ion at m/e 429 was used to monitor the two drugs. The sensitivity was 2 ng/ml for both drugs, which was sufficient to determine plasma and urine concentrations after therapeutic doses to humans.
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