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  • Title: The organization of the seminiferous epithelium in the mouse testis following ligation of the efferent ductules. A light microscopic study.
    Author: Ross MH.
    Journal: Anat Rec; 1974 Dec; 180(4):565-79. PubMed ID: 4440876.
    Abstract:
    In order to more fully assess and determine the relationship between the developing germ cells and the Sertoli cell epithelium, efferent ductules of Swiss albino mice testes were ligated and the effects observed. This method stretched the walls of the seminiferous tubules and thus reduced the stratification and complexity of the epithelium. At 48 hours postoperation, the testes were removed in a manner to prevent the escape of accumulated fluid. A marked size difference between the ligated and sham-operated testes was noted. Tissues were fixed in 3% phosphate buffered glutaraldehyde solution and later prepared for sectioning and examination by the light microscope. The seminiferous tubules in the ligated testes had relatively large lumens which contained spermatozoa, and the tubule epithelium was reduced in height. The various stages of cellular associations of the cycle of the epithelium were retained. Defined aggregations of germ cells grouped in specific association with the Sertoli cell elements were observed. The epithelium assumed the form of a series of parallel ridges at right angles to the tubules. Longitudinal sections of the tubules revealed pillarlike epithelial profiles. Each pillar consisted of Sertoli cell cytoplasm together with 2 generations of spermatids. The older generation of spermatids was embedded within the Sertoli cell and the younger generation along the sides. It is suggested that each generation within a ridge constitutes a single clone. The cytoplasmic bridges joining the spermatids and their attachments to the Sertoli cells are thought to determine the organization and structure of the rid ges. Several illustrations show the histological details of the structure.
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