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  • Title: The effect of systematic error on the accuracy of Michaelis constants and maximum velocities estimated by using the integrated Michaelis-Menten equation.
    Author: Newman PF, Atkins GL, Nimmo IA.
    Journal: Biochem J; 1974 Dec; 143(3):779-81. PubMed ID: 4462757.
    Abstract:
    Systematic errors in initial substrate concentration (s(0)), product concentration and reaction time give much larger errors in the Michaelis-Menten parameters unless s(0) is treated as an unknown parameter. These errors are difficult to detect because the fitted curve deviates little from the data. The effect of non-enzymic reaction is also examined.
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