These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Intercellular transport of steroids in the infused rabbit testis.
    Author: Van Doorn LG, De Bruijn HW, Galjaard H, Van der Molen HJ.
    Journal: Biol Reprod; 1974 Feb; 10(1):47-53. PubMed ID: 4462817.
    Abstract:
    Tritiated-pregnenolone and tritiated-testosterone were infused via the testicular artery into the rabbit testis in situ, in order to determine if steroids can pass the "blood-testis barrier". After various periods of infusion (5-60 minutes) the testis were frozen cryostat sections were cut and freeze-dried. Interstitium and tubules were isolated by micro dissection and radioactivity per mcg dry weight was measured in both tissue compartments. Radioactive steroids could be isolated from the interstitial tissue as well as from the seminiferous tubules. Levels of radioactivity in the testes after infusion of tritiated-pregnenolone varied from 4 to 50% of the infused dose and were found to be dependent on the type of perfusion medium and the duration of the perfusion. Separation and identification of the radioactive steroids after infusing tritiated prognenolone showed that pregnenolone and testosterone were the major radioactive steroids in both interstitium and seminiferous tubules. After infusion with tritiated-testosterone, both tritiated-testosterone (77%) and tritiated-androstenedione (23%) were dound in the seminiferous tubules. It is concluded that steroids can be transported from the Leydig cells to the seminiferous tubules.
    [Abstract] [Full Text] [Related] [New Search]