These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Excretion of deoxyribonucleic acid by lymphocytes stimulated with phytohemagglutinin or antigen.
    Author: Rogers JC, Boldt D, Kornfeld S, Skinner A, Valeri CR.
    Journal: Proc Natl Acad Sci U S A; 1972 Jul; 69(7):1685-9. PubMed ID: 4505646.
    Abstract:
    When human lymphocytes are cultured in the presence of phytomitogens, 70-90% of the cells undergo blast transformation and synthesize DNA. However, less than 40% of these lymphocytes actually undergo mitosis while 35-90% of the newly synthesized DNA is excreted into the media. The release of DNA by the cells is selective since experiments with [(14)C]uridine indicate that RNA is not lost into the culture media. DNA excretion occurs under many culture conditions. The excreted DNA has an estimated molecular weight of 3 to 12 x 10(6) as determined by gel filtration on Sepharose 2B. It forms a single sharp peak at a density of 1.055 g/cm(3) when examined by sucrose density gradient centrifugation, suggesting that the DNA is complexed to protein or lipid.
    [Abstract] [Full Text] [Related] [New Search]