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  • Title: DNA polymerase of chicken embryo: purification and properties.
    Author: Stavrianopoulos JG, Karkas JD, Chargaff E.
    Journal: Proc Natl Acad Sci U S A; 1972 Jul; 69(7):1781-5. PubMed ID: 4505656.
    Abstract:
    The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn(2+) ion for activity, and may contain Zn. It is very sensitive to oxidation.
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