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  • Title: Synthesis of a brain-specific protein (S100 protein) in a homologous cell-free system programmed with cerebral polysomal messenger RNA.
    Author: Zomzely-Neurath C, York C, Moore BW.
    Journal: Proc Natl Acad Sci U S A; 1972 Aug; 69(8):2326-30. PubMed ID: 4506102.
    Abstract:
    Polyribosomes, carrying nascent polypeptide chains, were prepared from whole brain, cortex, and hindbrain-medullary white matter of young adult rats. In a homologous cell-free system, a brain-specific protein (S100 protein) was identified in the mixture of polypeptides released from the polyribosomes during incubation for 1 hr at 37 degrees . De novo synthesis of the S100 protein was achieved in a reconstituted cerebral cell-free system containing polysome-derived mRNA and 40S + 60S subunits. The radioactively labeled S100 protein synthesized in vitro was identified by precipitation with antibody to S100 after addition of purified S100 as a carrier, and migration of the solubilized precipitate on acrylamide gels in the presence of sodium dodecyl sulfate. In vitro synthesis of the S100 protein did not occur in analogous cell-free systems derived from hepatic tissue or in a heterologous system containing liver polyribosomes and cerebral enzymes.
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