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  • Title: Formation and decay of prelumirhodopsin at room temperatures.
    Author: Busch GE, Applebury ML, Lamola AA, Rentzepis PM.
    Journal: Proc Natl Acad Sci U S A; 1972 Oct; 69(10):2802-6. PubMed ID: 4507603.
    Abstract:
    We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.
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