These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light.
    Author: Chu EH, Sun NC, Chang CC.
    Journal: Proc Natl Acad Sci U S A; 1972 Nov; 69(11):3459-63. PubMed ID: 4508333.
    Abstract:
    Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.
    [Abstract] [Full Text] [Related] [New Search]