These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Partial characterization of a tetrodotoxin-binding component from nerve membrane.
    Author: Benzer TI, Raftery MA.
    Journal: Proc Natl Acad Sci U S A; 1972 Dec; 69(12):3634-7. PubMed ID: 4509325.
    Abstract:
    Tetrodotoxin from Japanese puffer fish has been labeled with tritium and purified from the crude mixture obtained. The interaction between the purified [(3)H]tetrodotoxin and membrane suspensions from the olfactory nerve of long-nosed garfish has been investigated by equilibrium dialysis. Tetrodotoxin binds to membrane suspensions with a dissociation constant K(D) = 8.3 nM. The nerve preparation binds 42 pmol of [(3)H]tetrodotoxin/g of wet tissue at saturating toxin concentrations. With various hydrolytic enzymes, the binding component is shown to be a protein embedded in a phospholipid environment. The binding is inhibited below pH 4.0 and is not stable towards heat. Tetrodotoxin binding is not inhibited by the local anesthetic, procaine.
    [Abstract] [Full Text] [Related] [New Search]