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  • Title: Stability of cytoplasmic messenger RNA in HeLa cels.
    Author: Murphy W, Attardi G.
    Journal: Proc Natl Acad Sci U S A; 1973 Jan; 70(1):115-9. PubMed ID: 4509644.
    Abstract:
    The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [(3)H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.
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