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  • Title: Role of phospholipids in the multiple forms of mammalian elongation factor 1.
    Author: Legocki AB, Redfield B, Liu CK, Weissbach H.
    Journal: Proc Natl Acad Sci U S A; 1974 Jun; 71(6):2179-82. PubMed ID: 4526204.
    Abstract:
    Evidence is presented that high-molecular-weight aggregates of elongation factor 1 from calf brain and liver contain phospholipids. Sucrose gradient analysis has shown that these high-molecular-weight species can be converted to a low-molecular-weight form after incubation with different phospholipase preparations. In addition, a partially purified elongation factor 1 from rabbit reticulocytes is also converted to a low-molecular-weight species by phospholipase treatment. These data indicate that phospholipids are involved in maintaining the integrity of the high-molecular-weight species and support the view that this species is an aggregate of the low-molecular-weight elongation factor 1.
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