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  • Title: Equilibrium sedimentation of turnip yellow mosaic virus.
    Author: McGee JH, Kupke DW, Godschalk W, Beams JW.
    Journal: Proc Natl Acad Sci U S A; 1974 Oct; 71(10):3866-8. PubMed ID: 4530267.
    Abstract:
    Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 x 10(6) daltons was calculated for the virus at vanishing concentrations, in essential agreement with the earlier results using the more tedious procedure with a freely coasting rotor. In order to interpret the observed departure from ideal behavior (nonassociating conditions), preferential interaction experiments were carried out by magnetic densimetry. These showed that a strong Donnan effect exists at pH 7, which contributed > 10(3) times more to the nonideality than did the excluded volume effect. A net negative charge of about 3.5 x 10(3) per particle was estimated at this pH.
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