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  • Title: The immune response of HgA-negative rabbits to HgA-positive red cells treated with N-ethylmaleimide.
    Author: Smith GN, Mollison PL.
    Journal: Immunology; 1974 May; 26(5):877-83. PubMed ID: 4546949.
    Abstract:
    HgA-positive red cells were treated in vitro with approximately 10 μmoles N-ethylmaleimide (NEM) per ml cells for 1 hour at 37° and then injected into the circulation of HgA-negative rabbits; all the cells were cleared within 24 hours. After 1–3 months the animals were given a series of injections of untreated HgA-positive red cells at 3-monthly intervals. Five out of seventeen rabbits showed progressively diminishing survival of HgA-positive red cells with the development of anti-HgA. On the other hand, in eight rabbits red cell survival following three consecutive injections of untreated red cells was normal or almost normal and in four further rabbits survival was initially subnormal but became progressively longer and was almost normal following a third injection of cells. Thus, in as many as twelve out of seventeen animals (70 per cent) the survival of HgA-positive red cells following a third injection of untreated cells was normal or almost normal whereas this pattern was previously observed in only seven out of fifty-one animals (14 per cent) not given an initial injection of NEM-treated cells. It is concluded that the injection of NEM-treated HgA-positive red cells to HgA-negative rabbits induces a degree of immunological tolerance to the HgA antigen.
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