These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Peripheral blood T and B lymphocytes in patients with thyrotoxicosis and Hashimoto's thyroiditis and in normal subjects. A comparison of lymphocyte separation methods.
    Author: Urbaniak SJ, Penhale WJ, Irvine WJ.
    Journal: Clin Exp Immunol; 1974 Dec; 18(4):449-59. PubMed ID: 4549626.
    Abstract:
    Lymphocytes were separated from the peripheral blood of three groups of subjects (normal controls, untreated thyrotoxicosis and confirmed Hashimoto's thyroiditis) by two separation methods: dextran sedimentation and Ficoll–Triosil gradient centrifugation. It has been shown that there is a selective loss of T lymphocytes (as measured by sheep red cell rosettes) with relative enrichment of B lymphocytes (as measured by surface immunoglobulins) in the Ficoll–Triosil-separated suspensions. This distortion of the T/B ratio was seen to a similar extent in each of the three groups of subjects. Furthermore, the mean percentage T and B lymphocytes of both patient groups were not significantly different from those of the controls when separated by the same method. Optimal E-rosette formation occurred after prolonged incubation at 4°C and in the absence of serum. Direct counts using Toluidine Blue were superior to indirect counts with unstained rosette suspensions.
    [Abstract] [Full Text] [Related] [New Search]