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  • Title: Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.
    Author: Achtman M, Willetts N, Clark AJ.
    Journal: J Bacteriol; 1972 Jun; 110(3):831-42. PubMed ID: 4555412.
    Abstract:
    A series of 102 transfer-deficient (tra(-)) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F'/F') heterozygote cells in transient populations, and for measuring complementation between different Flac tra(-) mutants. These techniques were used to define nine tra cistrons and to assign most of the tra(-) mutations to one or other of these.
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